Lysis:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Protease Inhibitor:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Northern Blot:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Pore Size:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Fluorescence:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Microscopy:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Software:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
Spectrophotometry:Article Title: Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling
Article Snippet: A-21058 and A-21084) NP-40 lysis buffer (Thermo Scientific, cat. no. 28324) Protease inhibitor cocktail set II (EMD Chemicals, cat. no. 539132) Phosphatase inhibitor cocktail set II (EMD Chemicals, cat. no. 524625) DMEM (Gibco/BRL, cat. no. 11965118) FBS (HyClone, cat. no. SH30070.03) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l-glutamine (Invitrogen, cat. no. 25-005-CI) Penicillin/streptomycin (10,000 IU/10,000 μg; Invitrogen, cat. no. 30-002-CI) Milli-Q ultrapure water EQUIPMENT Lipid Extruder (1.5 ml; Northern Lipid) Rotary Evaporator (Buchi) equipped with a vacuum pump Polycarbonate filters (100 nm pore size; Whatman) Cell scrapers (Fisher Scientific) Nikon Eclipse TE2000-E inverted fluorescence microscope Stabilite 2018 krypton/argon ion laser (Spectra-Physics) Model 177 argon ion laser (Spectra-Physics) Quantix charge-coupled device (CCD) camera (Roper Scientific) Cascade 512B electron-multiplying CCD camera (Roper Scientific) Metamorph image acquisition software (Molecular Devices) Cy5, TR, NBD/HPTS, DAPI/Hoechst/AMCA, RICM filter cubes (Chroma Technology) Physitemp TS-4 thermal microscope stage (Physitemp Instruments) Oil immersion objective heater (100×; Home-built) Teflon cover slip mini-rack (Molecular Probes, cat. no. C-14784) Model 75D Aquasonic bath sonicator (VWR Instruments) Model 100CB CEE spin coater (Brewer Science) Model PC-400D hot plate (Corning) Dektak 150 + surface profiler (Veeco Instruments) Bioscope atomic force microscope (AFM) system (Digital Instruments) Edwards EB3 electron-beam evaporator (Edwards BOC) CABL-9510CC high-resolution electron beam lithography system (Crestec) Gemini Ultra-55 analytical scanning electron microscope (SEM; Zeiss) 90Plus particle size analyzer (Brookhaven Instruments) Odyssey infrared scanning system (LI-COR Biotechnology) Attofluor cell chamber (Invitrogen) Glass microscope cover slips (round, number 2, 170 μm thick, 25 mm in diameter; Fisher Scientific, cat. no. 12-546-2) Cary 100 scan UV-visible spectrophotometer (UV-visible; Varian) ImageJ software (http://rsbweb.nih.gov/ij/) REAGENT SETUP PBS, 1× It is diluted using DI water from a 10× PBS stock.
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